Regulatory

Part:BBa_K2418001:Design

Designed by: Akos Sukosd   Group: iGEM17_Szeged_SA_RMG   (2017-10-02)


Methylococcus capsulatus promoter - an improved version of BBa_K730001 - 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9
    Illegal BglII site found at 318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We could not know the exact sequence of the promoter, so we had to use the whole region and we had to use a restriction site not found in the sequence (BglII)


Source

Genomic sequence: https://www.ebi.ac.uk/ena/data/view/AE017282&display=fasta

References